Water quality. Determination of the estrogenic potential of water and waste water - In vitro human cell-based reporter gene assay has-corrigendum validation and routine monitoring and

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Water quality. Determination of the estrogenic potential of water and waste water - In vitro human cell-based reporter gene assay has-corrigendum validation and routine monitoring and1 Scope This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay utilizing stably transfected human cells. This reporter gene assay is based on the activation of the human estrogen receptor alpha. This method is applicable to: fresh water; waste water; aqueous extracts and leachates; eluates of sediments (fresh water); pore water; aqueous solutions of single